Behavioural tracking software
Applications > Open field
The open field test is about emotionality and locomotion, so automating it requires adaptable zone design and a comprehensive selection of locomotor related results.
Automating the open field test with ANY-maze is straightforward
and provides results that would be difficult, if not impossible,
to determine with simple observation and hand scoring - see
the Benefits tab, below, to learn more.
On the other tabs you'll find videos of open field tests, recommended equipment and a list of results that are especially useful in this test.
|Benefits||Useful results||Videos||Recommended equipment||Publications|
User defined zones
In ANY-maze you define zones that are specific to how you
want to perform tests in your apparatus - nothing's prescribed.
In this example video, zones have been set up to to score how much time the animal spends in the corners, how much time close to the walls and how much time in the centre of the enclosure.
In fact, for every zone ANY-maze reports more than 50 different measures, so you'll learn much more than just how long the animal spent in each location.
Going beyond zones
As well as scoring the animal's behaviour in zones, ANY-maze can also detect things such as:
Scoring behaviours software can't detect
You may be interested in how much time the animals spend grooming
in the open field; however this is a behaviour which software is
unable to reliably detect. To address this you can use a key to
score this behaviour; press the key while the animal's grooming and
release it when it stops. ANY-maze will then report information such
as time spent grooming and the number of grooming bouts, and it will report
this separately for each zone.
Of course, you're not limited to scoring grooming, you can use keys to score as many behaviours as you want.
Rearing is another behaviour often scored in the open field but which
(we believe) software is unable to reliably detect from top-down video.
However, rearing can be detected using an array of photo-beams positioned
such that when the animal rears it breaks beams in the array.
We manufacture an array of this type designed specifically for use with ANY-maze - it's pictured on the right.
When this array is utilised in the open field you'll get reports for (amongst other things) the number of rears in the different zones you define.
Viewing the animal's track
ANY-maze can plot the animal's track as a simple line (as
shown on the left) or as a heat map (shown on the right).
Heat maps, which indicate how much time the animal spent in different parts of the apparatus, can either show data for individual tests (as here) or averaged data for different groups.
In this example, the heat map makes it clear that the animal spent most time in the corners.
Simultaneously tracking in multiple apparatus
Tracking simultaneously in, for example, four open fields is a great way
to speed up the throughput in an experiment.
Almost everything can be changed
In ANY-maze you can alter the definition of a zone, a sequence or indeed almost anything
else, at any time, whether before, during or after you’ve run your tests.
For example, the size of the centre zone in the open field is somewhat debatable. Some researchers minimize it, while others define it as half of the overall test arena. If you ran an experiment with a large centre zone, but then wanted to see what would change with a smaller one, you could simply alter the size, and ANY-maze would immediately re-calculate the results to take this change into account.